Product Description

HSP47 Monoclonal Antibody

Product: Mouse IgG1. Supplied in crude ascites with 0.01% sodium azide.

Catalogue Numbers: MB66959-50, MB66959-100

Sizes: 50µl, 100µl

Swiss-Prot: P50454

Host: Mouse

Reactivity: Human, Mouse

Applications: WB, IHC

Background: Serpin peptidase inhibitor, clade H, member 1 (serpin H1), also known as heat shock protein 47 (HSP47), is a collagen specific molecular chaperone localized to the endoplasmic reticulum, essential for collagen biosynthesis. Despite its lack of serine proteinase activity, serpin H1 is a member of the serpin superfamily . Genetic deletion of SERPINH1 or inhibition of its interaction with procollagen has been shown to greatly reduce procollagen secretion from fibroblasts, implicating serpin H1 as a potential target for therapeutic management of fibrosis . Moreover, altered serpin H1 expression has been seen in multiple cancer types, including colon carcinoma, head and neck squamous cell carcinoma, and liver hepatocellular carcinoma. Thus, serpin H1 may function as a potential prognostic biomarker for certain cancers .

Product: Mouse IgG1. Supplied in crude ascites with 0.01% sodium azide.

Specificity: Recognizes endogenous levels of HSP47 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of HSP47 expression in rat heart (A) whole cell lysates., Immunohistochemical analysis of HSP47 staining in human skin carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 46 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: CBP1; CBP2; HSP47; SERPINH2; Serpin H1; 47 kDa heat shock protein; Arsenic-transactivated protein 3; AsTP3; Cell proliferation-inducing gene 14 protein; Collagen-binding protein; Colligin; Rheumatoid arthritis-related antigen RA-A47

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HSP47. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified