HSP60 Rabbit Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS9942M-50, BS9942M-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P10809
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
All Applications: WB: 1:1000-1:5000
IHC: 1:50-1:200
Background: HSPs (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins, and the HSP 110 family. The constitutively expressed mitochondrial protein HSP 60 shares the ability to recognize and stabilize proteins during folding, assembly and disassembly with other HSP family members. The mitochondrial and cytosolic localization of HSP 60, combined with its binding and catalysis of folding of newly synthesized proteins destined for the mitochondrial matrix, classify this protein as a molecular chaperone. An additional role of HSP 60 is to act as a cell surface marker forr γ/δ T cell recognition.
Purification and Purity: Protein A affinity purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: This antibody detects endogenous levels of HSP60 and does not cross-react with related proteins.
Bioworld Molecular Weight: ~ 60 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of HSP60 Rabbit mAb at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: MCF7 whole cell lysate
Lane 3: PANC-1 whole cell lysate
Lane 4: SW480 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-HSP60 antibody.Counter stained with hematoxylin.
Alternative Name: 60 kDa heat shock protein, mitochondrial; 60 kDa chaperonin; Chaperonin 60; CPN60; Heat shock protein 60; HSP-60; Hsp60; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein; HSPD1; HSP60
Immunogen: Recombinant antibody.
Conjugate: Unconjugated
Modification: Unmodified