HSP70 Mouse mAb - EM1042

HSP70 Mouse mAb

Sizes: 50μL, 100μL

Catalogue Numbers: EM1042-50, EM1042-100

Citations, Manuals and MSDS Available upon request.

Background: The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed heat shock proteins. Proteins with similar structure exist in virtually all living organisms. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. Hsp70 is usually in an ATP bound state. Hsp70 by itself is characterized by a very weak ATPase activity, such that spontaneous hydrolysis will not occur for many minutes. As newly synthesized proteins emerge from the ribosomes, the substrate binding domain of Hsp70 recognizes sequences of hydrophobic amino acid residues, and interacts with them. This spontaneous interaction is reversible, and in the ATP bound state Hsp70 may relatively freely bind and release peptides. However, the presence of a peptide in the binding domain stimulates the ATPase activity of Hsp70, increasing its normally slow rate of ATP hydrolysis.

Alternate Name: Heat shock 70 kDa protein 1/2, heat shock 70kDa protein 1A, HSP70, HSP70 1, HSP70 1/HSP70 2, HSP70 1A, HSP70.1/HSP70.2, HSP70I, HSP72, HSPA1, HSPA1A, HSPA1B

Source: Mouse

Applications: WB, IHC, IF

Dilution: WB 1:1, 000-2, 000 IF 1:100-200  IHC 1:200-500

Reactivity: Human, Rat, Mouse

Immunogen: Synthetic Peptide

Storage and Stability: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Clonality: Monoclonal

Isotype: IgG1

Concentration: 1mg/mL

Observed Band (KD): 70kDa

Human Gene ID: 3303

Human SWISS Prot NO: P0DMV8

Subcellular Location: Cytoplasm

Research Use Only