HSP70 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90669-50, BS90669-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P0DMV8
Host: Rabbit
Reactivity: Human, Mouse, rat
Applications: WB, ICC IHC
All Applications: WB:1:500
ICC:1:200
IHC:1:200
Background: The 70 kilodalton heat shock proteins (Hsp70s) are a family of conserved ubiquitously expressedheat shock proteins. Proteins with similar structure exist in virtually all living organisms. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. When not interacting with a substrate peptide, Hsp70 is usually in an ATP bound state. Hsp70 by itself is characterized by a very weak ATPase activity, such that spontaneous hydrolysis will not occur for many minutes. As newly synthesized proteins emerge from the?ribosomes, the substrate binding domain of Hsp70 recognizes sequences of hydrophobic amino acid residues, and interacts with them. This spontaneous interaction is reversible, and in the ATP bound state Hsp70 may relatively freely bind and release peptides. However, the presence of a peptide in the binding domain stimulates the ATPase activity of Hsp70, increasing its normally slow rate of ATP hydrolysis.
Purification and Purity: Peptide affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: HSP70 polyclonal antibody detects endogenous levels of HSP70 protein.
Bioworld Molecular Weight: 70 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of HSP70 on different cell lysates using anti-HSP70 antibody at 1/500 dilution.
Positive control:
Lane 1: A549
Lane 2: MCF-7
Lane 3: HCT116, ICC staining HSP70 in SHG-44 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: HSP70-1, HSP70.1, DnaK type molecular chaperone HSP70 1, Epididymis secretory protein Li 103, FLJ54303, FLJ54370, FLJ54392, FLJ54408, FLJ75127, Heat shock 70 kDa protein 1, Heat shock 70 kDa protein 1/2, Heat shock 70 kDa protein 1A/1B, Heat shock 70kDa protein 1A, Heat shock 70kDa protein 1B, Heat shock induced protein, HEL S 103, HSP70 1, HSP70 1B, HSP70 2, HSP70-1/HSP70-2, HSP70-1A, HSP70.1, HSP70.1/HSP70.2, HSP70I, HSP71, HSP72, HSPA1, HSPA1A, HSPA1B,
Immunogen: peptide
Conjugate: Unconjugated
Modification: Unmodified