HSP70 (Y41) Polyclonal Antibody-BS2741

HSP70 (Y41) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2741-50, BS2741-100

Sizes: 50µl, 100µl

Swiss-Prot: P08107

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: The HSP 70 family is composed of four highly conserved proteins: HSP 70, HSC 70, GRP 75 and GRP 78. These proteins serve a variety of roles: they act as molecular chaperones facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus and aid in the proper folding of nascent polypeptide chains. All members of the family, except HSP 70, are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70 play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery and are found in both the cytosol and nucleus of mammalian cells. Both HSP 70 and HSC 70 are involved in the chaperoning of nascent polypeptide chains and in protecting cells against the accumulation of improperly folded proteins.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: HSP70 (Y41) polyclonal antibody detects endogenous levels of HSP 70 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of HSP70 (Y41) polyclonal antibody at 1:500 dilution
Lane 1: MCF-7 whole cell lysate (40ug)
Lane 2: NIH-3T3 whole cell lysate (40ug)
Lane 3: PC12 whole cell lysate (40ug)
Lane 4: HEK293T whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of HSP70 (Y41) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 70 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2; HSPA1A; HSPA1; HSX70; HSPA1B; HSP-70; HSP 70

Immunogen: Synthetic peptide, corresponding to amino acids 20-70 of Human HSP 70.

Conjugate: Unconjugated

Modification: Unmodified