Product Description

HSP90B Mouse Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB9019-50, MB9019-100

Product: Mouse IgG2a, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P08238

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:5000
IHC: 1:100-1:200

Background: HSP 90 family members guide the normal folding, intracellular disposition and proteolytic turnover of many key regulators of cell growth, differentiation and survival. HSP 90alpha, also designated HSP90A, HSP 86 and LPS-associated protein 2 (LAP2), is a cytosolic enhancer of inducible nitric-oxide synthase (iNOS), with chaperone activity that is important for the transcriptional activity of p53. HSP 90beta, also designated HSP90B, HSP 84 and HSPC2, is a cytosolic protein that participates in signaling pathways with PKC epsilon to protect cells from external damage, particularly in heat shock-mediated events. GRP 94, also known as tumor rejection antigen 1 (TRA1), ECGP and GP96, localizes to the ER, is highly expressed in BGC-823 human gastric carcinoma cells and is upregulated in human endothelial cells in response to hypoxia by HIF-1.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of HSP90B and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 90 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of HSP90B Mouse mAb at 1:5000 dilution
Lane 1: Hela whole cell lysate
Lane 2: NIH/3T3 whole cell lysate
Lane 3: PC12 whole cell lysate
Lane 4: 293T whole cell lysate
Lane 5: HepG2 whole cell lysate
Lane 6: COS-1 whole cell lysate
Lane 7: Jurkat whole cell lysate
Lane 8: A549 whole cell lysate
Lane 9: Raji whole cell lysate
Lane 10: The heart tissue lysate of Mouse, Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-HSP90B antibody. Counter stained withhematoxylin.

Alternative Name: Heat shock protein HSP 90-beta; HSP 90; Heat shock 84 kDa; HSP 84; HSP84; HSP90AB1; HSP90B; HSPC2; HSPCB

Immunogen: This antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to HSP90B.

Conjugate: Unconjugated

Modification: Unmodified