Product Description

HuB/HuD Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16364-50, BZ16364-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: Q12926/P26378

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF, ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

Background: Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al. 1996 [PubMed 8663286]).

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 40 kDa; Observed MW: 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of HuB/HuD in Hela lysates using HuB/HuD antibody., Immunofluorescence analysis of HuB/HuD in HepG2 using ELAV2/4 antibody. The picture on the right is blocked using the synthesized peptide., Western blot analysis of HuB/HuD in Jurkat and HUVEC lysates using ELAV2/4 antibody. The Lane on the right is blocked with the synthesized peptide., Western blot analysis of HuB/HuD in 293 lysates using HuB/HuD antibody

Alternative Name: ELAVL2; HUB; ELAV-like protein 2; ELAV-like neuronal protein 1; Hu-antigen B; HuB; Nervous system-specific RNA-binding protein Hel-N1; ELAVL4; HUD; PNEM; ELAV-like protein 4; Hu-antigen D; HuD; Paraneoplastic encephalomyelitis antigen HuD

Immunogen: The antiserum was produced against synthesized peptide derived from human ELAV2/4. AA range:61-110

Conjugate: Unconjugated

Modification: Unmodified