Product Description

Human ACTN4 (Actinin Alpha 4) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK4564-48, ELK4564-96, ELK4564-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: FSGS; FSGS1; F-actin cross-linking protein; Non-muscle alpha-actinin 4

Assay Type: Sandwich

Sensitivity: 0.32 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: Developmental science;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ACTN4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACTN4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ACTN4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ACTN4 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: O43707

Research Use Only