Human CRP (C-Reactive Protein) ELISA Kit
Sizes: 24T, 48T, 96T
Catalogue Numbers: E-OSEL-H0002-24, E-OSEL-H0002-48, E-OSEL-H0002-96
Citations, Manuals and MSDS Available upon request.
Abbreviation: CRP
UNIProt ID: P02741
Target Synonyms: C-Reactive Protein; CRP; PTX1;
Research Areas: Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism, Immunology
Target Species: Human
Application: ELISA
Usage: This ELISA kit applies to the in vitro quantitative determination of Human CRP concentrations in serum, plasma.
Tested Sample Types: Serum, Plasma
Type: Sandwich
Assay Time: 1.5h
Detection Type: Colormetric
Sensitivity: 52.1 pg/mL
Detection Range: 125-8000 pg/mL
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CRP. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human CRP are added to the micro ELISA plate wells. Human CRP in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human CRP.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human CRP. The concentration of Human CRP in the samples is then determined by comparing the OD of the samples to the standard curve.
Cross Reactivity: This kit recognizes Human CRP in samples. No significant cross-reactivity or interference between Human CRP and analogues was observed
Recovery Rate: 80%-120%
Precision: Both intra-CV and inter-CV are < 10%.
Storage: 2-8℃, 6 months
Shipping: ice bag