Human CYP1A2 (Cytochrome P450, family 1, subfamily A, polypeptide 2) CLIA Kit - E-CL-H0297

Human CYP1A2 (Cytochrome P450, family 1, subfamily A, polypeptide 2) CLIA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-CL-H0297-24T, E-CL-H0297-48T, E-CL-H0297-96T

Citations, Manuals and MSDS Available upon request.

Abbreviation: CYP1A2

UNIProt ID: P05177

Target Synonyms: CP12; P3-450; P450(PA) ;

Research Areas: Cancer, Cardiovascular, Metabolism, Signal transduction

Target Species: Human

Application: CLIA

Usage: This ELISA kit applies to the in vitro quantitative determination of Human CYP1A2 concentrations in Tissue homogenates.

Tested Sample Types: Tissue homogenates

Type: Sandwich

Assay Time: 3.5h

Detection Type: Chemiluminescence

Sensitivity: 18.75 pg/mL

Detection Range: 31.25-2000 pg/mL

Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CYP1A2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CYP1A2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CYP1A2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human CYP1A2. You can calculate the concentration of Human CYP1A2 in the samples by comparing the RLU value of the samples to the standard curve.

Cross Reactivity: This kit recognizes Human CYP1A2 in samples. No significant cross-reactivity or interference between Human CYP1A2 and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃/-20℃, 6 months

Shipping: ice bag