Human FE (Ferritin) ELISA Kit
Sizes: 24T, 48T, 96T
Catalogue Numbers: E-OSEL-H0003-24, E-OSEL-H0003-48, E-OSEL-H0003-96
Citations, Manuals and MSDS Available upon request.
Abbreviation: FE
UNIProt ID: P02792,P02794
Target Synonyms: Ferritin; FE;
Research Areas: Cancer, Cardiovascular, Metabolism, Neuroscience, Signal transduction
Target Species: Human
Application: ELISA
Usage: This ELISA kit applies to the in vitro quantitative determination of Human FE concentrations in serum, plasma.
Tested Sample Types: Serum, Plasma
Type: Sandwich
Assay Time: 1.5h
Detection Type: Colormetric
Sensitivity: 24.31 pg/mL
Detection Range: 62.5-4000 pg/mL
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FE. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human FE are added to the micro ELISA plate wells. Human FE in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human FE.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human FE. The concentration of Human FE in the samples is then determined by comparing the OD of the samples to the standard curve.
Cross Reactivity: This kit recognizes Human FE in samples. No significant cross-reactivity or interference between Human FE and analogues was observed
Recovery Rate: 80%-120%
Precision: Both intra-CV and inter-CV are < 10%.
Storage: 2-8℃, 6 months
Shipping: ice bag