Human GnRH (Gonadotropin Releasing Hormone) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK5424-48, ELK5424-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: GNRH1; GRH; LNRH; LHRH; LH-RH I; Luliberin; Luteinizing-Hormone Releasing Hormone; Progonadoliberin-1; Gonadoliberin I; Gonadorelin; GnRH-associated peptide 1
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 5.42 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids
Assay length: 2h
Research Areas: Endocrinology; Reproductive science; Neuroscience; Genetic science; Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GnRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GnRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GnRH in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P01148
Research Use Only.