Human HLA-B27 (Human Leukocyte Antigen B27) CLIA Kit - E-CL-H0147

Human HLA-B27 (Human Leukocyte Antigen B27) CLIA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-CL-H0147-24T, E-CL-H0147-48T, E-CL-H0147-96T

Citations, Manuals and MSDS Available upon request.

Abbreviation: HLA-B27

UNIProt ID: P01889

Target Synonyms: Major Histocompatibility Complex (human) ; Class I; B27;

Research Areas: Immunology

Target Species: Human

Application: CLIA

Usage: This ELISA kit applies to the in vitro quantitative determination of Human HLA-B27 concentrations in serum, plasma and other biological fluids.

Tested Sample Types: Serum, Plasma and other biological fluids

Type: Sandwich

Assay Time: 3.5h

Detection Type: Chemiluminescence

Sensitivity: 37.5 pg/mL

Detection Range: 62.5-4000 pg/mL

Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HLA-B27. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HLA-B27 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HLA-B27, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human HLA-B27. You can calculate the concentration of Human HLA-B27 in the samples by comparing the RLU value of the samples to the standard curve.

Cross Reactivity: This kit recognizes Human HLA-B27 in samples. No significant cross-reactivity or interference between Human HLA-B27 and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃/-20℃, 6 months

Shipping: ice bag