Human HMGB-1 (High Mobility Group Protein B1) CLIA Kit - E-CL-H0997

Human HMGB-1 (High Mobility Group Protein B1) CLIA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-CL-H0997-24, E-CL-H0997-48, E-CL-H0997-96

Citations, Manuals and MSDS Available upon request.

Abbreviation: HMGB-1

UNIProt ID: P09429

Target Synonyms: HMGB1; HMG1; HMG3; SBP-1; Amphoterin;

Research Areas: Cancer, Epigenetics and Nuclear Signaling

Target Species: Human

Application: CLIA

Usage: This ELISA kit applies to the in vitro quantitative determination of Human HMGB-1 concentrations in serum, plasma and other biological fluids.

Tested Sample Types: Serum, Plasma and other biological fluids

Type: Sandwich

Assay Time: 3.5h

Detection Type: Chemiluminescence

Sensitivity: 7.5 pg/mL

Detection Range: 12.5-800 pg/mL

Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HMGB-1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human HMGB-1. You can calculate the concentration of Human HMGB-1 in the samples by comparing the RLU value of the samples to the standard curve.

Cross Reactivity: This kit recognizes Human HMGB-1 in samples. No significant cross-reactivity or interference between Human HMGB-1 and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃/-20℃, 6 months

Shipping: ice bag