Human LRP6 (Low Density Lipoprotein Receptor Related Protein) CLIA Kit - E-CL-H0791

Human LRP6 (Low Density Lipoprotein Receptor Related Protein) CLIA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-CL-H0791-24, E-CL-H0791-48, E-CL-H0791-96

Citations, Manuals and MSDS Available upon request.

Abbreviation: LRP6

UNIProt ID: O75581

Research Areas: Cancer, Signal transduction, Stem cells

Target Species: Human

Application: CLIA

Usage: This ELISA kit applies to the in vitro quantitative determination of Human LRP6 concentrations in Serum, plasma and other biological fluids.

Tested Sample Types: Serum, Plasma and other biological fluids

Type: Sandwich

Assay Time: 3.5h

Detection Type: Chemiluminescence

Sensitivity: 37.5 pg/mL

Detection Range: 62.5-4000 pg/mL

Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LRP6. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LRP6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LRP6, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human LRP6. You can calculate the concentration of Human LRP6 in the samples by comparing the RLU value of the samples to the standard curve.

Cross Reactivity: This kit recognizes Human LRP6 in samples. No significant cross-reactivity or interference between Human LRP6 and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃/-20℃, 6 months

Shipping: ice bag