Human MEP1a (Meprin A Alpha) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK5367-48, ELK5367-96, ELK5367-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: PPHA; PABA Peptide Hydrolase; Endopeptidase-2; N-benzoyl-L-tyrosyl-P-amino-Benzoic Acid Hydrolase Subunit Alpha; PABA Peptide Hydrolase
Assay Type: Competitive Inhibition
Sensitivity: 17 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.5-4000 pg/mL
Sample Type: Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Areas: Metabolic pathway
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MEP1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MEP1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MEP1a in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q16819
Research Use Only