Product Description

Human MIA1 (Melanoma Inhibitory Activity Protein 1) CLIA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-CL-H1409-24, E-CL-H1409-48, E-CL-H1409-96

Citations, Manuals and MSDS Available upon request.

Abbreviation: MIA1

UNIProt ID: Q16674

Research Areas: Cancer,Immunology,Signaling transduction

Target Species: Human

Application: CLIA

Usage: This ELISA kit applies to the in vitro quantitative determination of Human MIA1 concentrations in Serum, plasma and other biological fluids.

Tested Sample Types: Serum, Plasma and other biological fluids

Type: Sandwich

Assay Time: 3.5h

Detection Type: Chemiluminescence

Sensitivity: 4.69 pg/mL

Detection Range: 7.81-500 pg/mL

Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MIA1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MIA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MIA1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human MIA1. You can calculate the concentration of Human MIA1 in the samples by comparing the RLU value of the samples to the standard curve.

Cross Reactivity: This kit recognizes Human MIA1 in samples. No significant cross-reactivity or interference between Human MIA1 and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃/-20℃, 6 months

Shipping: ice bag