Human NIPA1 (Non Imprinted In Prader Willi/Angelman Syndrome Region Protein 1) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK5358-48, ELK5358-96, ELK5358-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: FSP3; SPG6; Magnesium transporter NIPA1; Spastic Paraplegia 6 (Autosomal Dominant)
Assay Type: Sandwich
Sensitivity: 0.057 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Areas: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NIPA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NIPA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NIPA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NIPA1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q7RTP0
Research Use Only