Human NTRK2 (Neurotrophic Tyrosine Kinase Receptor Type 2) ELISA Kit - ELK5214

Human NTRK2 (Neurotrophic Tyrosine Kinase Receptor Type 2) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK5214-48, ELK5214-96, ELK5214-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: GP145-TrkB; TRK-B; BDNF/NT-3 growth factors receptor; Tropomyosin-related kinase B; TrkB tyrosine kinase

Assay Type: Sandwich

Sensitivity: 0.056 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: Neuroscience

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NTRK2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NTRK2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NTRK2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NTRK2 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q16620

Research Use Only