Product Description

Human Pg (Progesterone) ELISA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-OSEL-H0011-24, E-OSEL-H0011-48, E-OSEL-H0011-96

Citations, Manuals and MSDS Available upon request.

Abbreviation: Pg

UNIProt ID: ???

Target Synonyms: Progesterone; Pg;

Research Areas: Signal Transduction

Target Species: Human

Application: ELISA

Usage: This ELISA kit applies to the in vitro quantitative determination of Human Pg concentrations in serum, plasma.

Tested Sample Types: Serum, Plasma

Type: Competitive

Assay Time: 1.5h

Detection Type: Colormetric

Sensitivity: 36.24 pg/mL

Detection Range: 78.13-5000 pg/mL

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human Pg are added to the micro ELISA plate wells. Human Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human Pg in the samples is then determined by comparing the OD of the samples to the standard curve.

Cross Reactivity: This kit recognizes Human Pg in samples. No significant cross-reactivity or interference between Human Pg and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃, 6 months

Shipping: ice bag