Human PKCa (Protein Kinase C Alpha) ELISA Kit - ELK4684

Human PKCa (Protein Kinase C Alpha) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK4684-48, ELK4684-96, ELK4684-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: PRKCA; PKC-A; AAG6; PKC-Alpha

Assay Type: Sandwich

Sensitivity: 12.2 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research Areas: Signal transduction; Enzyme and Kinase; Neuroscience;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PKCa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PKCa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PKCa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PKCa in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P17252

Research Use Only