Product Description

Human PRF1 (Perforin 1/Pore-Forming Protein) CLIA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-CL-H0749-24, E-CL-H0749-48, E-CL-H0749-96

Citations, Manuals and MSDS Available upon request.

Abbreviation: PRF1; PFP

UNIProt ID: P14222

Research Areas: Cancer, Immunology

Target Species: Human

Application: CLIA

Usage: This ELISA kit applies to the in vitro quantitative determination of Human PRF1/PFP concentrations in Serum, plasma and other biological fluids.

Tested Sample Types: Serum, Plasma and other biological fluids

Type: Sandwich

Assay Time: 3.5h

Detection Type: Chemiluminescence

Sensitivity: 7.5 pg/mL

Detection Range: 12.5-800 pg/mL

Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PRF1/PFP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PRF1/PFP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PRF1/PFP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human PRF1/PFP. You can calculate the concentration of Human PRF1/PFP in the samples by comparing the RLU value of the samples to the standard curve.

Cross Reactivity: This kit recognizes Human PRF1/PFP in samples. No significant cross-reactivity or interference between Human PRF1/PFP and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃/-20℃, 6 months

Shipping: ice bag