Human Smad4/MADH4 (Mothers Against Decapentaplegic Homolog 4 )CLIA Kit - E-CL-H0637

Human Smad4/MADH4 (Mothers Against Decapentaplegic Homolog 4 )CLIA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-CL-H0637-24, E-CL-H0637-48, E-CL-H0637-96

Citations, Manuals and MSDS Available upon request.

Abbreviation: Smad4; MADH4

UNIProt ID: Q13485

Research Areas: Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism, Signal transduction, Stem cells

Target Species: Human

Application: CLIA

Usage: This ELISA kit applies to the in vitro quantitative determination of Human Smad4/MADH4 concentrations in Serum, plasma and other biological fluids.

Tested Sample Types: Serum, Plasma and other biological fluids

Type: Sandwich

Assay Time: 3.5h

Detection Type: Chemiluminescence

Sensitivity: 37.5 pg/mL

Detection Range: 62.5-4000 pg/mL

Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Smad4/MADH4. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Smad4/MADH4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Smad4/MADH4, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human Smad4/MADH4. You can calculate the concentration of Human Smad4/MADH4 in the samples by comparing the RLU value of the samples to the standard curve.

Cross Reactivity: This kit recognizes Human Smad4/MADH4 in samples. No significant cross-reactivity or interference between Human Smad4/MADH4 and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃/-20℃, 6 months

Shipping: ice bag