Human SRY (Sex Determining Region Y) ELISA Kit - ELK4907

Human SRY (Sex Determining Region Y) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK4907-48, ELK4907-96, ELK4907-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: TDF; TDY; Testis-Determining Factor

Assay Type: Sandwich

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: Reproductive science;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SRY. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SRY. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SRY, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SRY in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q05066

Research Use Only