Human VASPIN (Visceral Adipose Specific Serine Protease Inhibitor) ELISA Kit - E-EL-H1762

Human VASPIN (Visceral Adipose Specific Serine Protease Inhibitor) ELISA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-EL-H1762-24, E-EL-H1762-48, E-EL-H1762-96

Citations, Manuals and MSDS Available upon request.

Abbreviation: VASPIN

UNIProt ID: Q8IW75

Target Synonyms: SERPINA12; OL-64; Visceral Adipose Tissue-Derived Serine Protease Inhibitor; Serine Proteinase Inhibitor; Clade A Member 12;

Research Areas: Cell Biology, Cardiovascular, Metabolism, Neuroscience, Signal transduction

Target Species: Human

Application: ELISA

Usage: This ELISA kit applies to the in vitro quantitative determination of Human VASPIN concentrations in serum, plasma and other biological fluids.

Tested Sample Types: Serum, Plasma and other biological fluids

Type: Sandwich

Assay Time: 3.5h

Detection Type: Colormetric

Sensitivity: 37.5 pg/mL

Detection Range: 62.5-4000 pg/mL

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VASPIN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VASPIN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VASPIN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VASPIN. You can calculate the concentration of Human VASPIN in the samples by comparing the OD of the samples to the standard curve.

Cross Reactivity: This kit recognizes Human VASPIN in samples. No significant cross-reactivity or interference between Human VASPIN and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃/-20℃, 12 months

Shipping: ice bag