Human WFDC5 (WAP Four Disulfide Core Domain Protein 5) ELISA Kit - ELK4487

Human WFDC5 (WAP Four Disulfide Core Domain Protein 5) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK4487-48, ELK4487-96, ELK4487-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: WAP1; Putative protease inhibitor WAP1; p53-responsive gene 5 protein

Assay Type: Sandwich

Sensitivity: 35 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Length: 3.5h

Research Areas: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human WFDC5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human WFDC5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human WFDC5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human WFDC5 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q8TCV5

Research Use Only