Human WNT7A (Wingless Type MMTV Integration Site Family, Member 7A) ELISA Kit - ELK6214

Human WNT7A (Wingless Type MMTV Integration Site Family, Member 7A) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK6214-48, ELK6214-96, ELK6214-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Proto-Oncogene Wnt7a Protein

Assay Type: Sandwich

Sensitivity: 11.2 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human WNT7A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human WNT7A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human WNT7A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human WNT7A in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: O00755

Research Use Only