Product Description

HUS1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS71950-50, BS71950-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O60921

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IF

All Applications: WB 1:500 - 1:2000 IF 1:50 - 1:200

Background: The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: HUS1 polyclonal antibody detects endogenous levels of HUS1 protein.

Bioworld Molecular Weight: 35kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using HUS1 antibody at 1:1000 dilution., Immunofluorescence analysis of U20S cell using HUS1 antibody. Blue: DAPI for nuclear staining., Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug HUS1 antibody at a dilition of 1:1000.

Alternative Name: Checkpoint protein HUS1;
hHUS1;
HUS1 (S. pombe) checkpoint homolog;
Hus1;
HUS1 checkpoint homolog (S. pombe);
HUS1 Checkpoint Protein;
HUS1 protein;
HUS1+ - like protein;
HUS1_HUMAN;
Hydroxyurea-sensitive 1, S. pombe, homolog of;

Immunogen: Recombinant protein of human HUS1

Conjugate: Unconjugated

Modification: Unmodified