HXK I Rabbit Monoclonal Antibody-BS9927M

HXK I Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9927M-50, BS9927M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P19367

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB: 1:1000-1:2000
IHC/ICC/IF: 1:50-1:200
FC: 1:50-1:100

Background: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of HXK I and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 102 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of HXK I Rabbit mAb at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: 293 whole cell lysate
Lane 3: MCF7 whole cell lysate
Lane 4: HepG2 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Hexokinase 1 antibody. Counter stained with hematoxylin., ICC staining Hexokinase 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Hexokinase-1; Hexokinase 1; Brain form hexokinase; Hexokinase type I; HK I; HK1; HXK1; HXK 1

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified