Product Description

IBA1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90681-50, BS90681-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P55008

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P IF/ICC

All Applications: WB 1:1:500-1:1000
ICC/IF 1:50-1:200
IHC-P 1:50-1:100

Background: Ionized calcium-binding adapter molecule 1 (Iba1), also known as allograft inflammatory factor-1 (AIF-1), is a 147 amino acid cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. Iba1, containing two EF domains, is induced by cytokines and interferons. In an unstimulated state, Iba1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding Iba1 maps to chromosome 6p21.33 and resides in the tumor necrosis factor (TNF) cluster of genes located in the region represented by the human major histocompatibility complex (MHC).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at +4°Cafter thawing. Aliquot store at -20°Cor -80°CAvoid repeated freeze / thaw cycles.

Specificity: IBA1 polyclonal antibody detects endogenous levels of IBA1 protein.

Bioworld Molecular Weight: ~ 17 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of THP-1 cells, using IBA1 antibody at 1:1000 dilution., Immunofluorescence analysis of rat brain cells using IBA1 pAb at dilution of 1:50 (40x lens).

Alternative Name: AIF1; AIF-1; IBA1; IRT-1; IRT1; Iba1

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human AIF1/IBA1 (NP_001614.3).

Conjugate: Unconjugated

Modification: Unmodified