ICA1 Polyclonal Antibody Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Sizes: 50µl, 100µl Catalogue Numbers: BS77291-50, BS77291-100 Swiss-Prot: Q05084 Host: Rabbit ICA1 Polyclonal Antibody-BS77291 Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Reactivity: Human Applications: WB, IHC All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 Background: This gene encodes a protein with an arfaptin homology domain that is found both in the cytosol and as membrane-bound form on the Golgi complex and immature secretory granules. This protein is believed to be an autoantigen in insulin-dependent diabetes mellitus and primary Sjogren' s syndrome. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2013] Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Specificity: Unmodification Bioworld Molecular Weight: 69kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Western blot analysis of extracts of various cell lines, using ICA1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s., Immunohistochemistry of paraffin-embedded Human colon using ICA1 Rabbit pAb at dilution of 1:100.Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol. Alternative Name: ICA69; ICAp69; ICA1 Immunogen: Recombinant fusion protein of human ICA1(NP_001263407). Modification: Unmodified Conjugate: Unconjugated