Product Description

ICAD (Q177) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2229-50, BS2229-100

Sizes: 50µl, 100µl

Swiss-Prot: O00273

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: The CED/ICE family of cysteine proteases plays a pivotal role in mediating apoptosis through the proteolysis of specific targets. Among the targets are poly (ADP-ribose) polymerase (PARP), gelsolin, DFF-45/ICAD and the nuclear lamins. PARP is a 112 kDa nuclear protein that is specifically cleaved by CPP32 and Mch2, but not by ICE, into a signature 85 kDa apoptotic fragment. Gelsolin is cleaved by CPP32 to an active form that severs actin filaments in a Ca++-independent manner. In addition to binding actin, gelsolin can form complexes with fibronectin, which may be important for localizing gelsolin to inflammatory sites. DFF-45/ICAD, the 45 kDa subunit of DNA fragmentation factor, is cleaved by CPP32 to generate an active factor that induces DNA fragmentation. The 70 kDa nuclear Lamin A is cleaved by Mch2, but not CPP32. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: ICAD (Q177) polyclonal antibody detects endogenous levels of ICAD protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of IDCAD (Q177) polyclonal antibody at 1:500 dilution
Lane 1: C6 whole cell lysate (40ug)
Lane 2: CT26 whole cell lysate (40ug)
Lane 3: HCT116 whole cell lysate (40ug)
Lane 4: COS-7 whole cell lysate (40ug)
Lane 5: SGC7901 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of ICAD (Q177) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 36 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: DNA fragmentation factor subunit alpha; DNA fragmentation factor 45 kDa subunit; DFF-45; Inhibitor of CAD; ICAD; DFFA; DFF1; DFF45; H13

Immunogen: Synthetic peptide, corresponding to amino acids 151-200 of Human ICAD.

Conjugate: Unconjugated

Modification: Unmodified