Product Description

ICAM-1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90682-50, BS90682-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P05362

Host: Rabbit

Reactivity: Human

Applications: WB, ICC IHC FC

All Applications: WB:1:500-1:1,000
ICC:1:200
IHC:1:200
FC:1:50-1:100

Background: ICAM-1 is a member of the immunoglobulin superfamily, the superfamily of proteins including antibodies and T-cell receptors. The structure of ICAM-1 is characterized by heavy glycosylation, and the protein’s extracellular domain is composed of multiple loops created by disulfide bridges within the protein. ICAM-1 can be induced by interleukin-1 (IL-1) and tumor necrosis factor (TNF) and is expressed by the vascular endothelium, macrophages, and lymphocytes. ICAM-1 is a ligand for LFA-1 (integrin), a receptor found on leukocytes. More recently, ICAM-1 has been characterized as a site for the cellular entry of human rhinovirus. ICAM-1 and soluble ICAM-1 have antagonistic effects on the tight junctions forming the blood-testis barrier, thus playing a major role in spermatogenesis. ICAM-1 has been implicated in subarachnoid hemorrhage (SAH).

Purification and Purity: Peptide affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: ICAM-1 polyclonal antibody detects endogenous levels of ICAM-1 protein.

Bioworld Molecular Weight: 90 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of ICAM1 on different cell lysates using anti- ICAM1 antibody at 1/500 dilution.
Positive control:
Lane 1: Raji
Lane 2: HUVEC
Lane 3: K562, ICC staining of ICAM1 in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: ICAM-1, Antigen identified by monoclonal, BB2, BB 2, BB2, CD 54, CD_antigen=CD54, CD54, Cell surface glycoprotein P3.58, Human rhinovirus receptor, ICAM 1, ICAM-1, ICAM1, ICAM1, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, Intercellular adhesion molecule 1, Major group rhinovirus receptor, MALA 2, MALA2, MyD 10, MyD10, P3.58, Surface antigen of activated B cells, BB2,

Immunogen: peptide

Conjugate: Unconjugated

Modification: Unmodified