Product Description

ICOS/CD278 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6795-50, BS6795-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9Y6W8

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 30-40KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using ICOS/CD278 antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s., Immunofluorescence analysis of rat thymus using ICOS/CD278 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of human spleen using ICOS/CD278 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of mouse thymus using ICOS/CD278 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: ICOS; AILIM; CD278; CVID1

Immunogen: Recombinant fusion protein of human ICOS/CD278(NP_036224.1).

Conjugate: Unconjugated

Modification: Unmodified