Product Description

ID1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90683-50, BS90683-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P41134(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF, IHC, FC

All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Members of the Id family of basic helix-loop-helix (bHLH) proteins include Id1, Id2, Id3 and Id4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The Id-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since Id1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the Id protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the Id proteins is strongly dependent on growth factor activation and that reduction of Id mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: ID1 polyclonal antibody detects endogenous levels of ID1 protein.

Bioworld Molecular Weight: 22 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Id1 on 293T cell using anti-Id1 antibody at 1/1,000 dilution., ICC staining Id1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: bHLHb24, ID1, BHLHB24, ID, bHLHb24, Class B basic helix-loop-helix protein 24, dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein), DNA binding protein inhibitor ID 1, DNA binding protein inhibitor ID1, DNA-binding protein inhibitor ID-1, Dominant negative helix loop helix protein, ID 1, ID, ID1, ID1, Inhibitor of Differentiation 1, Inhibitor of DNA binding 1, inhibitor of DNA binding 1, dominant negative helix-loop-helix protein,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified