IDO2 Polyclonal Antibody
Catalogue Numbers: BS60857-50, BS60857-100
Sizes: 50µl, 100µl
Alternative Name: Indoleamine 2,3-dioxygenase 2 (EC:1.13.11.-); IDO-2; Indoleamine 2,3-dioxygenase-like protein 1; Indoleamine-pyrrole 2,3-dioxygenase-like protein 1; IDO2; INDOL1
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q6ZQW0
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: Tryptophan is an essential amino acid that is necessary for protein synthesis, serotonin and melatonin biosynthesis and energy production; energy being a product of the catabolism of tryptophan through the kynurenine pathway. The kynurenine pathway has many downstream metabolites which may be a part of physiological or patho-physiological processes. INDOL1 (indoleamine 2,3-dioxygenase-like protein 1) is an enzyme that catalyzes the first step of the kynurenine pathway of tryptophan metabolism. INDOL1 is also known as IDO2 (indoleamine 2,3-dioxygenase 2) and is a 407 amino acid protein that is expressed in various tissues, including liver, small intestine, spleen, placenta, thymus, lung, brain, kidney, colon and dendritic cells. INDOL1 is selectively inhibited by D-1MT (1-methyl-d-tryptophan), which also inhibits IDO (indoleamine 2,3-dioxygenase) and is significant because IDO expression causes suppression of T cell responses to tumors in dendritic cells. The inhibition of INDOL1 by D-1MT suggests a common function in immunomodulation. In the human INDOL1 gene, two single nucleotide polymorphisms have been detected which abolish the enzymatic function of INDOL1.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IDO2 polyclonal antibody detects endogenous levels of IDO2 protein.
Bioworld Molecular Weight: ~ 45 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of IDO2 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: PC12 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human IDO2.
Conjugate: Unconjugated
Modification: Unmodified