Product Description

IFI35 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS76759-50, BS76759-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Acts as a signaling pathway regμlator involved in innate immune system response. In response to interferon IFN-alpha, associates in a complex with signaling pathway regμlator NMI to regμlate immune response, the complex formation prevents proteasome-mediated degradation of IFI35 and correlates with IFI35 dephosphorylation. In complex with NMI, inhibits virus-triggered type I interferon/IFN-beta production. In complex with NMI, negatively regμlates nuclear factor NF-kappa-B signaling by inhibiting the nuclear translocation, activation and transcription of the NF-kappa-B subunit p65/RELA, resμlting in the inhibition of endothelial cell proliferation, migration and re-endothelialization of injured arteries. Beside its role as an intracellμlar signaling pathway regμlator, also functions extracellμlarly as damage-associated molecμlar patterns (DAMPs to promote inflammation when actively released by macrophage to the extracellμlar space during cell injury and pathogen invasion. Macrophage-secreted IFI35 activates NF-kappa-B signaling in adjacent macrophages through Toll-like receptor 4/TLR4 activation, thereby inducing NF-kappa-B translocation from the cytoplasm into the nucleus which promotes the release of proinflammatory cytokines.

Category: Primary Antibody

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB, IF/ICC

Alternate Names: IFI35, IFP35

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human IFI35(NP_005524.2).

Dilution: WB, 1:500 - 1:2000 IF/ICC, 1:50 - 1:100

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Unmodification

Molecular Weight: 35kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P80217

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only