IGF1 Polyclonal Antibody
Catalogue Numbers: BS60538-50, BS60538-100
Sizes: 50µl, 100µl
Alternative Name: IGF-I; Mechano growth factor; MGF; Somatomedin-C; IGF1; IBP1
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P05019
Host: Rabbit
Reactivity: Human, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: Insulin-like growth factor I, or IGF-I, is a ubiquitous peptide that acts in both an autocrine and paracrine fashion to stimulate the growth of vascular smooth muscle cells. In addition, IGF-I regulates renal function, growth and repair, is critically involved in bone formation and resorption and has been implicated in mediating aspects of the immune response. IGF function is modulated by at least six circulating IGF-binding proteins, designated IGFBP1-6, which associate with the soluble growth factor. While the function of IGF-II is less well understood, overexpression of the protein in mice suggests that IGF-II may play a regulatory role in insulin sensitivity and glucose uptake. Both IGF-I and IGF-II exert their biological effects through a common receptor, designated IGF-IR. Like the insulin receptor, IGF-IR is composed of two extracellular a chains and two signal transducing b chains cross-linked by disulfide bonds.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IGF1 polyclonal antibody detects endogenous levels of IGF1 protein.
Bioworld Molecular Weight: ~ 22 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of IGF1 polyclonal antibody at 1:500 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: PC12 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human IGF1
Conjugate: Unconjugated
Modification: Unmodified