IKBα (Phospho-S32) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS94018-50, BS94018-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P25963(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, ICC IP,
All Applications: WB:1:1,000
ICC:1:50-1:200
Background: On the basis of both functional and structural considerations, members of the IκB family of proteins can be divided into four groups. The first of these groups, IκB-α, includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NFkB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to κB sites, suggesting that the IκB-α family binds to the p65 subunit of p50-p65 heterocomplex through ankyrin repeats. The second member of the IκB family is represented by a protein designated IκB-β. The third group of IκB proteins is represented by IκB-γ, which is identical in sequence with the C-terminal domain of the p110 precursor of NFkB p50 and is expressed predominantly in lymphoid cells. An additional IκB family member, IκB-ε, has several phosphorylated forms and is primarily found complexed with Rel A and/or c-Rel.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: IKBα (Phospho-S32) polyclonal antibody detects endogenous levels of IKBα protein only when phosphorylated at S32.
Bioworld Molecular Weight: 36 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Phospho-IKB alpha(S32) on Ags cell lysates using anti-Phospho-IKB alpha(S32) antibody at 1/1,000 dilution., ICC staining Phospho-IKB alpha(S32) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: IkB-alpha, IkappaBalpha, NFKBIA, IKBA, MAD3, NFKBI, I kappa B alpha, I-kappa-B-alpha, IkappaBalpha, IkB-alpha, IKBA, IKBA, IKBalpha, MAD 3, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF kappa B inhibitor alpha, NF-kappa-B inhibitor alpha, NFKBI, NFKBIA, Nuclear factor of kappa light chain gene enhancer in B cells, Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha,
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser32 of human IKB alpha.
Conjugate: Unconjugated
Modification: Phosphorylation