IKBβ Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90694-50, BS90694-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q15653(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, ICC, IHC, IP,
All Applications: WB:1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
Background: On the basis of both functional and structural considerations, members of the IkB family of proteins can be divided into four groups. The first of these groups, IkB-α, includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NFkB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to kB sites, suggesting that the IkB-α family binds to the p65 subunit of p50-p65 heterocomplex through ankyrin repeats. The second member of the IkB family is represented by a protein designated IkB-β. The third group of IkB proteins is represented by IkB-ε, which is identical in sequence with the C-terminal domain of the p110 precursor of NFkB p50 and is expressed predominantly in lymphoid cells. An additional IkB family member, IkB-é, has several phosphorylated forms and is primarily found complexed with Rel A and/or c-Rel.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: IKBβ polyclonal antibody detects endogenous levels of IKBβ protein.
Bioworld Molecular Weight: 48 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of IKB beta on different lysates using anti-IKB beta antibody at 1/1,000 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: Hela
Lane 3: THP-1, ICC staining IKB beta in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: NF-kappa-BIB, IkB-B, IkB-beta, IkappaBbeta, TR-interacting protein 9, TRIP-9, NFKBIB, I kappa B beta, I-kappa-B-beta, IkappaBbeta, IKB beta, IkB-B, IkB-beta, IKBB, IKBB, IkBbeta, NF kappa BIB, NF-kappa-B inhibitor beta, NF-kappa-BIB, Nfkbib, Thyroid receptor interacting protein 9, Thyroid receptor-interacting protein 9, TR interacting protein 9, TR-interacting protein 9, TRIP-9, TRIP9,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified