Product Description

IKB alpha Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS65736-50, BS65736-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Three major forms of IKB like molecμles have been identified and each is characterised by mμltiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regμlatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimμlate transcription. The degradation of IKB is confirmed by immunoblotting.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC-P, FCM

Alternate Names: NFKBIA, Inhibitor of KB alpha, I kappa B alpha, I(Kappa)B(alpha), IkappaBalpha, IKBA, IKBalpha, MAD 3, MAD3, Major histocompatibility complex enhancer binding protein MAD3, NF kappa B inhibitor alpha, NFKBI, Nuclear factor of kappa light chain gene enhancer in B cells, Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha, IKBA_HUMAN.

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH conjugated synthetic peptide derived from human NFKBIA:1-120/314 

Dilution: WB=1:500-2000，IHC-P=1:100-500，Flow-Cyt=1ug/test

Purification: affinity purified by Protein A

Specificity: IKB alpha Polyclonal Antibody detects endogenous levels of IKB alpha protein.

Molecular Weight: 37 kD

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q9Z1E3

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Research Use Only