Product Description

IKKα/β (phospho-S176) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS5083-50, BS5083-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins. Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB. The key regμlatory step in this pathway involves activation of a high molecμlar weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regμlatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resμlting in kinase activation.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: IHC

Alternate Names: I kappa-B kinase alpha, IkBKA, IKK-alpha, IKK-A, IkappaB kinase, EC 2.7.11.10, I-kappa-B kinase 1, IKK1, Conserved helix-loop-helix ubiquitous kinase, Nuclear factor NF-kappa-B inhibitor kinase alpha, NFKBIKA, CHUK, IKKA, TCF16

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Dilution: IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-IKK-a/b (S176) polyclonal antibody detects endogenous levels of IKK-a/b protein when phosphorylated at Ser176.

Molecular Weight: ~ 85 kDa

Pathway: NF-kB Signaling, PI3K AKT signaling, Immune Cell Signaling, STING Signaling, Inhibition of Apoptosis,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O15111/O14920

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only