Product Description

IKKα/β (Phospho-S180/181) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: AP0625-50, AP0625-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins. Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB. The key regμlatory step in this pathway involves activation of a high molecμlar weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regμlatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resμlting in kinase activation.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB, IF

Alternate Names: Inhibitor of nuclear factor kappa-B kinase subunit alpha, I-kappa-B kinase alpha, IKK-A, IKK-alpha, IkBKA, IkappaB kinase, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase 1, IKK1, Nuclear factor NF-kappa-B inhibitor kinase alpha, NFKBIKA, Transcription factor 16, TCF-16, CHUK, IKKA, TCF16

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylated

Immunogen: Synthetic phosphopeptide derived from human IKKα/β around the phosphorylation site of Serine 180/181.

Dilution: WB: 1:2000~1:5000
IF: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: IKKα/β (Phospho-S180/181) polyclonal antibody detects endogenous levels of IKKα/β protein only when phosphorylated at Ser180/181.

Molecular Weight: ~ 75 kDa

Pathway: NF-kB Signaling, PI3K AKT signaling, Immune Cell Signaling, STING Signaling, Inhibition of Apoptosis,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O15111/O14920

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Research Use Only