Product Description

IKKβ (phospho-Y188) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4101-50, BS4101-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The transcription factor NFκB is retained in the cytoplasm in an inactive form by the inhibitory protein IκB. Activation of NFκB requires that IκB be phosphorylate on specific serine residues, which resμlts in targeted degradation of IκB. IκB kinase α (IKKα), previously designated CHUK, interacts with IκB-α and specifically phosphorylates IκB-α on Serines 32 and 36, the sites that trigger its degradation. IKKα appears to be critical for NFκB activation in response to proinflammatory cytokines. Phosphorylation of IκB by IKKα is stimμlated by the NFκB inducing kinase (NIK), which itself is a central regμlato for NFκB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ (also designated NEMO), and each appear to make essential contributions to IκB phosphorylation.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: Inhibitor of nuclear factor kappa B kinase beta subunit, I-kappa-B-kinase beta, IkBKB, IKKB, I-kappa-B kinase 2, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, NFKBIKB

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human IKKβ around the phosphorylation site of Tyrosine 188.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-IKKβ (Y188) polyclonal antibody detects endogenous levels of IKKβ protein only when phosphorylated at Tyr188.

Molecular Weight: ~ 86 kDa

Pathway: NF-kB Signaling, PI3K AKT signaling, Immune Cell Signaling, STING Signaling, Inhibition of Apoptosis, Toll-like Receptor signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O14920

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only