Product Description

IKKγ (D25) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: AP0384-50, AP0384-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins. Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB. The key regμlatory step in this pathway involves activation of a high molecμlar weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regμlatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resμlting in kinase activation. Activation of the NF-κB pathway by the T-cell lymphotrophic virus Tax protein or by TNF-α treatment leads to IKKβ-dependent phosphorylation of human IKKγ, primarily at Ser376. In mice, mutation of the orthologous residue (Ser369) to alanine leads to enhanced IKKγ-mediated stimlμlation of IKKβ kinase activity.

Category: Primary Antibody

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB, IF

Alternate Names: NF-kappa-B essential modμlator, NEMO, FIP-3, IkB kinase-associated protein 1, IKKAP1, Inhibitor of nuclear factor kappa-B kinase subunit gamma, I-kappa-B kinase subunit gamma, IKK-gamma, IKKG, IkB kinase subunit gamma, NF-kappa-B essential modifier, IKBKG, FIP3, NEMO

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: Synthetic peptide, corresponding to Human IKKγ.

Dilution: WB: 1:500~1:1000
IF: 1:100~1:500

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: IKKγ (D25) polyclonal antibody detects endogenous levels of IKKγ protein.

Molecular Weight: ~ 49 kDa

Pathway: NF-kB Signaling, STING Signaling, Inhibition of Apoptosis, Toll-like Receptor signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q9Y6K9

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Research Use Only