Product Description

IKKγ (Phospho-S31) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: AP0266-50, AP0266-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which resμlts in targeted degradation of IkB. IkB kinase α (IKKα), previously designated CHUK, interacts with IkB-α and specifically phosphorylates I˚Bα on Serine 32 and 36, the sites that trigger its degradation. IKKα appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKKα is stimμlated by the NFkB inducing kinase (NIK), which itself is a central regμlator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ (also designated NEMO), and each appear to make essential contributions to IkB phosphorylation.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB, IF, IHC

Alternate Names: NF-kappa-B essential modμlator, NEMO, FIP-3, IkB kinase-associated protein 1, IKKAP1, Inhibitor of nuclear factor kappa-B kinase subunit gamma, I-kappa-B kinase subunit gamma, IKK-gamma, IKKG, IkB kinase subunit gamma, NF-kappa-B essential modifier, IKBKG, FIP3, NEMO

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human IKKγ around the phosphorylation site of Serine 31.

Dilution: WB: 1:1000~1:2000
IHC: 1:50~1:200
IF: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: IKKγ (Phospho-S31) polyclonal antibody detects endogenous levels of IKKγ protein only when phosphorylated at Ser31.

Molecular Weight: ~ 54 kDa

Pathway: NF-kB Signaling, STING Signaling, Inhibition of Apoptosis,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q9Y6K9

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Research Use Only