IL-12B (V277) Polyclonal Antibody-BS3480

IL-12B (V277) Polyclonal Antibody

Catalogue Numbers: BS3480-50, BS3480-100

Sizes: 50µl, 100µl

Alternative Name: Interleukin-12 subunit beta; IL-12B; Cytotoxic lymphocyte maturation factor 40 kDa subunit; CLMF p40; IL-12 subunit p40; NK cell stimulatory factor chain 2; NKSF2; NKSF2; IL12B

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P29460

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: IL-12 is secreted by macrophages and human B-lymphoblastoid cells in response to antigenic stimulation. This IL is responsible for the differentiation of naive CD4+ T cells into type 1 helper T cells that produce interferon-γ (IFN-γ). It also activates production of tumor necrosis factor α (TNFα) from T and natural killer (NK) cells, and it inhibits IL-4 mediated suppression of IFN-γ. IL-12 also has anti-angiogenic activity since the production of IFN-γ increases the production of inducible protein-10 (IP-10). IL-12 p40 represents a subunit of IL-12 that is induced by several pathogens. IL-12 p70 is a subunit of IL-12 that is controlled through both the p35 and p40 genes in a cell type-specific fashion.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IL-12B (V277) polyclonal antibody detects endogenous levels of IL-12B protein.

Bioworld Molecular Weight: ~ 37 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of IL-12B (V277) polyclonal antibody at 1:500 dilution
Lane 1: Hela whole cell lysate(40ug)
Lane 2: K562 whole cell lysate(40ug)
Lane 3: CT26 whole cell lysate(40ug)
Lane 4: PC12 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 245-291 of Human IL-12B.

Conjugate: Unconjugated

Modification: Unmodified