IL-31 Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB9293-50, MB9293-100
Product: Mouse IgG3, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q6EBC2(Human)
Host: Mouse
Reactivity: Human
Applications: WB, IHC ICC
All Applications: WB:1:500
ICC:1:100
IHC:1:50-1:200
Background: IL-31 is a T cell cytokine that is preferentially produced by T helper type 2 cells. IL-31 signals through a heterodimeric receptor composed of the IL-31 receptor (IL-31R) and the oncostatin M receptor (OSM). This receptor complex recruits JAK1, JAK2, Stat1, Stat3 and Stat5 signaling pathways, as well as the PI3 kinase/AKT cascade. SHP-2 and Shc adapter molecules are also recruited and contribute to an increased activation of the MAP kinase pathway in response to IL-31. Overexpression of IL-31 in mice results in pruritus and skin dermatitis resembling human atopic dermatitis (AD). Comparisons between skin from patients with AD and healthy skin showed IL-31R expression at higher levels on epidermal keratinocytes in AD samples. Infiltrating cells, more numerous in skin from patients with AD compared with that of healthy individuals, expressed IL-31 mRNA. IL-31 may participate in the cause of itch sensation and promote scratching behavior in NC/Nga mice with atopic dermatitis, and may represent a novel target for antipruritic drug development.
Purification and Purity: Protein affinity purified.
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: IL-31 monoclonal antibody detects endogenous levels of IL-31 protein.
Bioworld Molecular Weight: 18 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of IL-31 on recombinant protein lysate using anti-IL-31 antibody at 1/500 dilution., ICC staining IL-31 (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: IL-31, IL 31, IL-31, IL31, IL31, Interleukin 31, Interleukin-31,
Immunogen: Recombinant full length protein corresponding to human IL18.
Conjugate: Unconjugated
Modification: Unmodified