Product Description

IL-32 (K226) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3676-50, BS3676-100

Sizes: 50µl, 100µl

Swiss-Prot: P24001

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Interleukin-32 (IL-32), also designated Natural Killer (NK) cell transcript 4 (nk4), may play a role in inflammatory responses such as lymphocyte activation. IL-32 is secreted following stimulation with inflammatory cytokines such as IL-1β and IFN-γ, and by NK cells after exposure to IL-12 and IL-18. It also induces production of IL-1β, TNFα, MIP-2 and IL-6. Expression of IL-32 increases after activation of T cells by mitogens or activation of NK cells by IL-2. The IL-32 protein contains three potential N-myristoylation sites, a tyrosine sulfation site, an RGD cellattachment sequence and multiple putative phosphorylation sites. Human IL-32 exists as four isoforms.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: IL-32 (K226) polyclonal antibody detects endogenous levels of IL-32 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of IL-32 (K226) pAb at 1:500 dilution
Lane 1: HepG2 whole cell lysate (40ug)
Lane 2: LO2 whole cell lysate (20ug)
Lane 3: SGC7901 whole cell lysate (40ug)
Lane 4: PMVEC whole cell lysate (40ug)
Lane 5: AML-12 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of IL-32 (K226) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 27 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Interleukin-32; IL-32; Natural killer cells protein 4; Tumor necrosis factor alpha-inducing factor; NK4; TAIF; IL32

Immunogen: Synthetic peptide, corresponding to amino acids 200-2450 of Human IL-32.

Conjugate: Unconjugated

Modification: Unmodified