Product Description

IL-4 Polyclonal Antibody

Catalogue Numbers: BS5764-50, BS5764-100

Sizes: 50µl, 100µl

Alternative Name: Interleukin-4; Interleukin 4; Interleukin4; B-cell stimulatory factor 1; BSF-1; BSF1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra; IL4

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P05112

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Interleukin-4 (IL-4), also designated B cell stimulatory factor-1, is a glycosylated cytokine secreted by activated T lymphocytes, basophils and mast cells. The secreted IL-4 protein promotes the growth and differentiation of cells that participate in immune defense by favoring such events as the expansion of the Th2 lineage relative to Th1 cells. These T helper cell subsets are defined by their pattern of cytokine secretion: Th1 cells secrete IL-2, TNFβ and IFN-γ, while Th2 cells secrete IL-4, IL-5 and IL-10. Another key immunological function of IL-4 is to induce immunoglobulin class switching. IL-4 has been shown to induce the production of IgE and enhance IgG4 secretion by B cells, but suppress the production of IgM, IgA, IgG1, IgG2, and IgG3.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IL-4 polyclonal antibody detects endogenous levels of IL-4 protein.

Bioworld Molecular Weight: ~ 18 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of IL-4 polyclonal antibody at 1:500 dilution
Lane 1: MCF-7 cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: PC12 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 108-153 of Human IL-4.

Conjugate: Unconjugated

Modification: Unmodified